Extracts of Adenopus breviflorus induce opening of rat liver mitochondrial membrane permeability transition pore

Tolulope Oyedeji, Tunmise Akintehinse, Erhunmwunsee Avan, Oluwaremi Soremekun, Oluwatumininu Solomon, Olufunso Olorunsogo


The induction of programmed cell death (apoptosis) through the modulation of Mitochondrial Membrane Permeability Transition (MMPT) pore signalling pathway by tropical medicinal plant extracts is useful in the treatment of tumours resistant to chemotherapy. Fortunately, nature has provided potential phytochemicals which are capable of inducing opening of the MMPT pore in cells where little or no apoptosis takes place. The effect of aqueous and methanol extracts of Adenopus breviflorus (AB) used locally against the outbreak of measles and chicken pox was assessed on rat liver MMPT pore. Sixty male albino rats weighing between 120 – 150g were used in this study. For in vivo studies, 50 rats of ten animals per group were assigned to five groups and treated with distilled water (control) and varying doses of the aqueous extract of Adenopus breviflorus (AEAB), (50, 100, 200 and 400 mg/kg respectively). Rat liver mitochondria were isolated by differential centrifugation and MMPT assayed spectrophotometrically.  The results obtained from in vitro studies revealed that calcium ion induced MMPT pore opening by 10.0 folds, however, spermine significantly reversed calcium-induced MMPT pore opening in rat liver mitochondrial by 85%. The result further revealed that the AEAB induced MMPT pore opening in a concentration dependent manner. The extract at 50, 150, 250 and 350µg/ml significantly induced pore opening by 1.0, 10.0, 13.0 and 18.0 folds respectively. In the presence of calcium, the AEAB further opened MMPT pore by 16.0, 17.0, 16.0 and 15.6 folds respectively. Similarly, the methanol extract of Adenopus breviflorus (MEAB), significantly induced MMPT pore opening by 0.4, 1.1 and 8.0 folds at 150, 250 and 350µg/ml respectively and further opened the MMPT pore in the presence of calcium by 10.8, 12.0, 10.4, and 10.3 folds at 50, 150, 250 and 350µg/ml respectively. The results obtained from the in vivo studies, revealed that, the oral administration of the AEAB for 7 days, significantly induced MMPT pore opening by 6.3 folds only at the dose of 400 mg/kg. Similarly, MMPT pore opening was significantly induced in animals orally exposed to the AEAB for 14days. Specifically, MMPT pore was induced by 8.0, 13.0, 8.0 and 25.0 folds at 50, 100, 200 and 400mg/kg.  These findings, suggest that potential phytochemicals that are capable of inducing apoptosis are present in the aqueous and methanol extracts of Adenopus breviflorus and these bioactive agents may serve as potential drug in the treatment of tumours.

Full Text:



  • There are currently no refbacks.