Attenuation of alloxan-induced disturbance of glucose metabolism, liver function and antioxidant status by methanol leaf extract of Synsepalum dulcificum



The effect of a methanol leaf extract of Synsepalum dulcificum on biochemical indices of hyperglycemia, oxidative stress and liver function in alloxan-induced diabetic rats was examined. Diabetes was induced by the administration of 125 mg/kg alloxan to Wistar rats. Animals with confirmed hyperglycemia were treated with 25 mg/kg MSD, 50 mg/kg MSD or 2 mg/kg glibenclamide and sacrificed after 14 days of treatment. Serum glucose concentration, activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) alkaline phosphatase (ALP) and superoxide dismutase (SOD) and levels of reduced glutathione (GSH), malondialdehyde and total protein were evaluated. Animals in the diabetic control group had a significantly higher glucose level compared with the normal control, extract- and glibenclamide-treated diabetic animals (P<0.05). The percentage weight loss in extract and glibenclamide treated diabetic animals was lower compared to the value for diabetic controls. Moreover, the diabetic control group had significantly higher activities of AST, ALT, and ALP compared with the normal control, extract and glibenclamide treated groups (P<0.05). Malondialdehyde level in the diabetic control animals was higher compared to normal control animals and animals in the treated groups (P<0.05) while the reverse pattern was observed for total protein level, GSH concentration and SOD activity. The results suggest that methanolic extract of Synsepalum dulcificum may be useful in combating diabetes mellitus.

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